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M94A3161.TXT
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1994-10-25
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Document 3161
DOCN M94A3161
TI The role of human CD4 and CD26 in HIV infection of murine and rabbit
transfectants.
DT 9412
AU Yamamura Y; Ikawa Y; Dept. Biochemistry, Tokyo Medical & Dental Univ.
Sch. Medicine,; Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):139 (abstract no. PA0176). Unique
Identifier : AIDSLINE ICA10/94369414
AB OBJECTIVE: Human CD4 is essential for HIV binding but not for HIV entry
into the cells to establish infection. Although the human CD26 molecule
was recently reported to be a cofactor that helps HIV infection, it is
unclear whether only human CD4 and CD26 are sufficient for HIV
infection. We evaluate the roles of human CD4 and CD26 in HIV-1 binding,
entry, replication, and infection using murine and rabbit T cells and
discuss the possibility of mice and rabbits as animal models for HIV
infection. METHODS: Human CD4 and CD26 cDNAs were inserted into an
expression vector BCMGSNeo and introduced into murine and rabbit T
cells. The transfectants expressing human CD4 and CD26 were examined for
susceptibility to HIV-1 by incubating with HIV-1 or by cocultivating
with HIV-1-infected MOLT-4 cells. RESULTS: Rabbit but murine
transfectans expressing human CD4 transiently expressed HIV-specific
antigens after the HIV-1 infection. After the cell-mediated HIV-1
infection, prominent syncytia formation together with the production of
the HIV capsid protein, p24 and budding virions was observed in rabbit
transfectants expressing human CD4 but was not in murine transfectants.
Furthermore, data will be presented using murine and rabbit
transfectants expressing both human CD4 and CD26. Preliminarily, murine
transfectants expressing both human CD4 and CD26 were not susceptible to
HIV-1 infection. DISCUSSION AND CONCLUSIONS: Our results suggest that
only human CD4 and CD26 are not sufficient for HIV infection. Rabbit T
cells might have or might be provided by MOLT-4 cells with the third
molecule which is required for HIV infection.
DE Animal Antigens, CD/BIOSYNTHESIS/*PHYSIOLOGY Antigens,
CD4/BIOSYNTHESIS/*PHYSIOLOGY Antigens, Differentiation,
T-Lymphocyte/BIOSYNTHESIS/*PHYSIOLOGY Cell Line Giant Cells Human
HIV-1/*PHYSIOLOGY Mice Rabbits Recombinant
Proteins/BIOSYNTHESIS/METABOLISM T-Lymphocytes/*IMMUNOLOGY/MICROBIOLOGY
*Transfection Viral Proteins/BIOSYNTHESIS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).